Collagen IV alpha2 Colorimetric Cell-Based ELISA Kit

A colorimetric cell-based ELISA kit from Assay Genie is designed for the qualitative detection of Collagen IV alpha2 protein expression in adherent cultured cells. The assay is reactive with human and mouse targets and is suited to cell culture supernatants, serum, or plasma samples. The manufacturer specifies a dynamic range of > 5000 Cells and a colorimetric detection method read at 450 nm, with storage at 4°C and a stated stability of 6 months.

Described as a lysate-free, high-throughput and sensitive assay, the kit uses an indirect ELISA format in which Collagen IV alpha2 is captured by specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. It can be used to screen for the effects of treatments, inhibitors such as siRNA or chemicals, or activators on Collagen IV alpha2. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, and Crystal Violet whole-cell staining may be used to determine cell density.

The procedure involves seeding adherent cells, fixation, quenching and blocking steps, overnight incubation with primary antibody at 4°C, followed by HRP-conjugated secondary antibody, ready-to-use substrate and stop solution, with absorbance read at 450 nm. The product is categorized under cardiovascular research applications.