Cofilin (Phospho-Ser3)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for the qualitative detection of Cofilin phosphorylation at serine 3, supporting research into cell signalling pathways and cytoskeletal dynamics. The assay targets Cofilin (Phospho-Ser3) and shows reactivity with Human, Mouse, and Rat samples, making it suitable for studies across multiple model systems and disease areas including cancer, inflammation, and neurodegenerative disorders.

The kit employs an indirect ELISA format in which Cofilin (Phospho-Ser3) is captured by phospho-specific primary antibodies and detected using HRP-conjugated secondary antibodies, producing a colour change read at 450 nm. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalisation, and Crystal Violet whole-cell staining can be used to determine cell density and adjust for plating differences. The assay is described as a high-throughput, lysate-free method for measuring relative phosphorylation in cultured cells and for screening the effects of treatments, inhibitors, or activators on Cofilin phosphorylation.

The kit is configured for a microplate-based workflow with a stated dynamic range of > 5000 Cells and is intended for use with adherent cell samples as well as serum, plasma, and lysate samples. The associated target is the CFL1 gene product (Gene ID: 3716; UniProt ID: P23528), Cofilin-1, an actin-binding protein that regulates actin depolymerization and is implicated in processes such as cell migration, adhesion, and cytokinesis.