CLK2 Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA Kit is designed for the precise measurement of CLK2 levels across various cell types and tissue samples. The kit is a convenient, lysate-free, high throughput and sensitive assay that can detect CLK2 protein expression profile in cells, allowing measurement of the relative amounts of CLK2 in cultured cells and screening for the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on CLK2.

Qualitative determination of CLK2 concentration is achieved by an indirect ELISA format: CLK2 is captured by CLK2-specific primary antibodies while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyse a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalising the target absorbance values, and following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, results can be analysed by normalising the absorbance values to cell amounts, by which plating differences can be adjusted.

Reactivity is reported for Human, Mouse and Rat samples, with a dynamic range of > 5000 Cells. The detection method is colorimetric, read at 450 nm, and the kit is suitable for adherent cells. Storage is at 4°C with 6 months stability. The kit is intended for research applications, offering easy-to-follow protocols and user-friendly procedures for studies of CLK2 biology and its implications in human health and disease.