Claudin 7 Colorimetric Cell-Based ELISA Kit

From Assay Genie, the Claudin 7 Colorimetric Cell-Based ELISA Kit is a high-quality in-cell ELISA kit designed for the precise measurement of Claudin-7 levels in cell samples. Using an indirect ELISA format, the kit captures Claudin 7 via specific primary antibodies and detects them with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition. It offers a sensitive, lysate-free, high-throughput approach for profiling Claudin 7 protein expression in adherent cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on Claudin 7.

The kit is reactive with Human, Mouse, and Rat samples, with a dynamic range of > 5000 Cells. Detection is colorimetric, read at 450 nm, and the assay is supplied in a 96-Well Microplate format. Storage stability is 4°C for 6 Months. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining can be used to determine cell density after the colorimetric read.

Claudin-7 is a key tight-junction protein in epithelial cells, central to cell barrier function and tissue integrity. Its dysregulation has been implicated in cancer, inflammatory bowel disease, and lung disorders, making it a valuable biomarker for studies of epithelial barrier function and disease mechanisms.