Claudin 3 (Phospho-Tyr219)Colorimetric Cell-Based ELISA Kit

Assay Genie offers a cell-based ELISA kit designed for the precise measurement of phosphorylated Claudin-3 levels in cell lysates. The kit targets the Claudin 3 (Phospho-Tyr219) phospho-specific site and is reactive with human samples. Phosphorylation of Claudin-3 at Tyr219 has been implicated in various diseases, including cancer and inflammatory disorders, making this a valuable tool for investigating tight junction biology and related signaling pathways.

The assay uses a colorimetric detection method read at 450 nm and follows an indirect ELISA format. Claudin 3 (Phospho-Tyr219) is captured by primary antibodies specific for the phospho site, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalyzing a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and a Crystal Violet whole-cell staining step is provided to determine cell density. The dynamic range is specified as "> 5000 Cells."

Claudin-3 is a key component of tight junctions in epithelial cells, regulating paracellular permeability and calcium-independent cell-cell adhesion. The protein is encoded by the CLDN3 gene (Gene ID: 1365, UniProt ID: O15551) and maps to chromosomal location 7q11.23. As a lysate-free, high-throughput format, the kit is suitable for screening the effects of various treatments, inhibitors, or activators on Claudin 3 phosphorylation in cultured cells.