CIM (Cimaterol) ELISA Kit

Cimaterol is a beta-2 adrenergic agonist from the clenbuterol family, illicitly administered to livestock as a repartitioning agent to enhance lean muscle deposition. Assay Genie's competitive immunoassay supports food safety laboratories conducting residue surveillance in beef, pork, and aquaculture supply chains. The microplate arrives pre-coated with Cimaterol-specific capture antibodies; sample analyte competes with an HRP-labelled Cimaterol conjugate, yielding an inverse colour intensity proportional to residue concentration.

Key specifications include a detection range of 0.05 to 4.05 ppb, sensitivity of 0.05 ppb, and a sample volume of 50 µL per well. Cross-reactivity validation shows negligible response to related beta-agonists such as clenbuterol, salbutamol, ractopamine, and terbutaline, allowing selective quantification of Cimaterol in urine, muscle, and serum matrices. Reconstituted standards and conjugate vials must be refrigerated and shielded from light, while unused wells remain sealed in the foil pouch with desiccant for later runs. The package contains a pre-coated microplate, six lyophilised standard vials, conjugate, TMB substrate, stop solution, concentrated wash buffer, and a comprehensive protocol insert for in vitro research use only.