Ciclosporin (CsA) ELISA Kit

From Assay Genie, this competitive ELISA kit is designed for the quantitative measurement of ciclosporin, also known as cyclosporine A, in biological samples. The detection method is colorimetric, with absorbance read at 450 nm on a microplate reader, and the kit is intended for research use only.

Ciclosporin, also known as cyclosporine A, is described as a potent immunosuppressive drug widely used in organ transplantation and autoimmune disease research, where monitoring ciclosporin levels is considered crucial to ensure therapeutic efficacy and prevent potential toxicity. The kit enables quantitative measurement that supports work on drug pharmacokinetics, dosing optimisation and patient compliance assessment. Manufacturer-supplied sample preparation protocols cover serum, plasma, urine, cerebrospinal fluid, cell culture supernatant, cell lysates, tissue homogenates, tissue lysates and breast milk, with the note that over-haemolysed samples are not suitable for use.

Measurement range is 3.12-200 ng/mL, with reactivity described as general. Equipment not supplied includes a microplate reader with a 450 nm filter, a multichannel pipette, an incubator and deionised or distilled water. Component storage conditions differ: the microplate strips, lyophilised standard, sample diluent, assay diluents and detection reagents are stored at -20°C, while wash buffer, substrate and stop solution are stored at 4°C.