Chk2 (Phospho-Thr387)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed to detect Chk2 (Phospho-Thr387) — the phosphorylated form of Checkpoint Kinase 2 at threonine 387 — directly in cultured cells. The assay uses an indirect ELISA format in which target-specific primary antibodies capture the phosphorylated protein and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition, read at 450 nm.

The kit is lysate-free and intended for use with adherent cells. It can be applied to measure relative levels of phosphorylated Chk2 in cultured cells as well as to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Chk2 phosphorylation. Reactivity is reported for Human, Mouse, and Rat samples. A dynamic range of > 5000 Cells is supported, and the determination is qualitative. Two normalization methods are provided: a monoclonal antibody specific for human GAPDH serves as an internal positive control, and following the colorimetric measurement, the Crystal Violet whole-cell staining method may be used to determine cell density so that absorbance values can be normalized to cell amounts.

The kit is supplied in a 96-well microplate format and is positioned for research into DNA damage response, cell cycle regulation, and cancer biology, where phosphorylation of CHK2 at Thr387 is known to activate its kinase activity in response to replication blocks and DNA damage.