Chk1 (Phospho-Ser286)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the accurate measurement of CHK1 phosphorylation at serine 286 in cell lysates. The kit offers a high throughput, lysate-free workflow and uses an indirect ELISA format in which target-specific primary antibodies capture the analyte and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition.

Reactivity is reported for human samples, with adherent cells as the sample type and a dynamic range of > 5000 Cells. Detection is by colorimetric readout at 450 nm. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and Crystal Violet whole-cell staining can be used to determine cell density after the colorimetric measurement.

The kit is intended for measuring relative amounts of phosphorylated Chk1 in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on Chk1 phosphorylation. CHK1 is described as a key regulator of cell cycle checkpoints and the DNA damage response, and phosphorylation at serine 286 is described as essential for its activation and function in response to DNA damage, making this a useful tool for studies of cellular stress, cancer biology, and potential therapeutic targets.