Chk1 Colorimetric Cell-Based ELISA Kit

Designed for researchers investigating DNA damage response and cell cycle regulation, this colorimetric cell-based ELISA kit from Assay Genie targets Chk1, a serine/threonine kinase involved in checkpoint-mediated cell cycle arrest and DNA repair. The kit is reactive with human, mouse, and rat samples, with detection performed at 450 nm using a colorimetric method. The assay is described as suitable for adherent cell samples and provides a convenient, lysate-free, high-throughput approach for measuring relative Chk1 protein expression in cultured cells.

The qualitative indirect ELISA format uses Chk1-specific primary antibodies captured on the plate, with HRP-conjugated secondary antibodies binding the Fc region to catalyze a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included to serve as an internal positive control for normalizing target absorbance values, while Crystal Violet whole-cell staining can be used to determine cell density and adjust for plating differences. The kit supports screening applications such as evaluating the effects of various treatments, inhibitors (including siRNA or chemicals), or activators on Chk1.

Database identifiers for the Chk1 target (gene symbol CHEK1) include Gene ID 1111, UniProt ID O14757, OMIM 603078, and Unigene Hs.24529. The target protein is reported with a molecular weight of 50,415 Da. The assay's stated dynamic range is > 5000 Cells, and storage stability is given as 4°C for 6 months. The kit is positioned for cell cycle research.