Chicken Troponin I, fast skeletal muscle (TNNI2) ELISA Kit

The TNNI2 gene encodes the fast-twitch skeletal muscle isoform of troponin I, a key regulatory protein of the striated muscle contractile apparatus. Measuring circulating TNNI2 levels by ELISA has become a valuable research tool in poultry science for evaluating muscle development, stress responses, and meat quality traits in chicken models.

Key features of this Assay Genie Sandwich ELISA include colorimetric detection at 450 nm using a standard microplate reader, a pre-coated 96-well plate, and compatibility with serum and plasma samples. Each kit provides reagents for 96 determinations, with calibrated standards included for quantitative analysis. Standard curves span a working range for detecting physiological and pathophysiological concentrations in avian samples.

For best results, store all reagents at 2-8 °C, protected from light, and avoid repeated freeze-thaw cycles. Bring samples and kit components to room temperature before use, and run the assay according to the included protocol. Intended for research use only in vitro applications, including muscle physiology research, veterinary biomarker studies, and investigations of avian muscle disease.

Troponin I fast-twitch skeletal muscle isoform (TNNI2) is encoded by the TNNI2 gene and acts as the inhibitory subunit of the heterotrimeric troponin complex on the thin filament of striated muscle. In chickens, this fast skeletal isoform is predominantly expressed in the pectoralis and leg muscles that drive rapid locomotion, so measuring circulating TNNI2 in serum offers a useful biomarker for evaluating muscle fibre integrity, exercise-induced stress, and growth performance in broiler and layer breeds.

Each Assay Genie Sandwich ELISA is supplied as a complete 96-well microplate kit containing a pre-coated capture antibody, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, stop solution, wash buffer, and a lyophilised recombinant chicken TNNI2 standard. The protocol typically follows a 1-2 hour room-temperature incubation, with absorbance read at 450 nm and a standard curve spanning approximately 0.156-10 ng/mL for quantitative interpolation of unknown serum or plasma samples. Recommended sample volumes are 50-100 µL per well after appropriate dilution in the provided assay buffer.

For poultry muscle biology and meat science workflows, this kit supports studies on muscle development, myopathy biomarkers (such as wooden breast and white striping), heat-stress responses, and the impact of nutritional or genetic interventions on skeletal muscle turnover. Store all unopened kit components at 2-8 °C, protect the TMB substrate from light, and limit the reconstituted standard to no more than three freeze-thaw cycles to preserve binding activity. Intended for research use only in vitro applications.