Chicken Transferrin receptor protein 1 (TFRC) ELISA Kit

Transferrin receptor protein 1 (TFRC), also known as CD71, is the primary cell-surface receptor for transferrin-bound iron and plays a central role in iron homeostasis, erythropoiesis, and cellular proliferation. This Sandwich ELISA from Assay Genie uses a colorimetric detection method read at 450 nm, enabling quantitative measurement of TFRC in chicken serum and plasma samples. Intended for research use only, the kit is supplied in a 96-well microplate format sufficient for 96 determinations. Standard storage at 2-8 °C preserves reagent activity, and the reagents remain stable for several months under refrigerated conditions. Applications include avian iron metabolism studies, poultry nutrition research, anemia models, investigations of immune cell activation, and characterization of proliferative responses in chicken models.

The Assay Genie kit uses a sandwich ELISA format that pairs a capture antibody pre-coated on the microplate with a biotinylated detection antibody, developed with streptavidin-HRP and TMB substrate for the chromogenic readout. Each well typically requires ~50 µL of sample, and a serial-dilution standard curve enables quantitative interpolation across a defined dynamic range. For consistent results, aliquot serum and plasma upon collection and store at -80 °C, avoiding repeated freeze-thaw cycles. Because chicken TFRC is upregulated on activated lymphocytes and erythroid progenitors, this assay complements flow cytometry and immunohistochemistry workflows in avian immunology, hematology, and vaccine-response studies.