Chicken Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) ELISA Kit

The Assay Genie Chicken TIMP3 ELISA Kit quantitatively measures endogenous TIMP3 in avian biological samples using a microplate-based immunoassay workflow. Tissue Inhibitor of Metalloproteinases 3 (TIMP3) is a secreted glycoprotein uniquely anchored to the extracellular matrix, where it regulates matrix metalloproteinase (MMP) activity, modulates angiogenesis, and influences inflammatory responses. In poultry research, TIMP3 quantification supports studies of fibrotic liver disease, ovarian follicular development, skeletal muscle growth, and cartilage formation in broiler and layer breeds.

Each kit provides a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, chromogenic substrate, wash buffer, and a recombinant chicken TIMP3 reference standard for constructing a calibration curve. The chicken-specific antibody pair minimizes cross-reactivity with other avian TIMP family members, supporting accurate measurement of endogenous TIMP3 rather than related isoforms. Researchers pipette standards and unknown samples, incubate per protocol, wash, and develop the color reaction for absorbance-based quantitation. Store kit components refrigerated upon arrival and protect the substrate from light.

Documented applications include monitoring plasma biomarkers in nutritional intervention trials, evaluating tissue remodeling after pathogen or vaccine challenge, and characterizing TIMP3 expression in primary chicken cell cultures. The assay is intended for research use only and supports comparative endocrinology, developmental biology, and avian disease model investigations in commercial and academic poultry laboratories.