Chicken Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (ATP2A1) ELISA Kit

The Assay Genie Chicken ATP2A1 sandwich ELISA is supplied as a 96-well microplate pre-coated with capture antibody specific to SERCA1, the fast-twitch skeletal muscle isoform of the sarcoplasmic/endoplasmic reticulum calcium ATPase. This colorimetric immunoassay enables quantitative detection of ATP2A1 in chicken serum and plasma samples across a working range suited to muscle physiology and poultry muscle biology research. Each kit includes the pre-coated plate, lyophilized standards, biotinylated detection antibody, concentrated wash buffer, TMB substrate, and stop solution sufficient for 96 determinations. Reagents should be stored at 2-8 °C, kept refrigerated, and protected from light to preserve antibody activity through the stated shelf life. The assay follows a standard sandwich ELISA workflow using approximately 100 µL of sample per well, and the resulting color change is read at 450 nm with a microplate reader, making the protocol straightforward for research use only laboratories investigating calcium handling, excitation-contraction coupling, and broiler skeletal muscle development.