Chicken Protein disulfide-isomerase (P4HB) ELISA Kit

The Assay Genie Sandwich ELISA Kit quantifies P4HB (also known as Protein Disulfide-Isomerase or PDI), a key endoplasmic reticulum enzyme that catalyzes the formation, breakage, and rearrangement of disulfide bonds during protein folding. The assay uses a pre-coated 96-well microplate, chicken-specific capture and detection antibodies, and a colorimetric readout typically measured at 450 nm. Serum and plasma samples are pipetted into wells alongside a standard curve, allowing researchers to interpolate unknown P4HB concentrations with strong sensitivity. Reagents should be stored refrigerated at 2-8 °C, with the microplate kept sealed and protected from light until use. The kit is intended for research use only, supporting studies in avian biology, oxidative protein folding, cellular stress responses in poultry, and investigations of chaperone function in commercial chicken lines.