Chicken Prostaglandin E2 (PGE2) ELISA Kit

Designed for the precise measurement of Prostaglandin E2 (PGE2) levels in chicken-derived samples, this competitive ELISA kit from Assay Genie supports research into inflammatory and immune responses in poultry. PGE2 is described as a key mediator of inflammation in chickens, playing a critical role in physiological processes such as fever, pain, and inflammation, and accurate quantification of this analyte can provide valuable insights into inflammatory status and potential therapeutic interventions in avian biology and veterinary medicine.

The assay is validated for use with serum and plasma samples, and is also described as applicable to tissue culture samples, using a user-friendly protocol and high-quality components. It measures PGE2 across a standard range of 15.6-1000 pg/mL. Kit components include a dismountable ELISA microplate, lyophilized standard, sample diluent, assay diluent A, assay diluent B, detection reagent A, detection reagent B, wash buffer, substrate solution, stop solution, and plate sealer; storage temperatures vary between -20°C and 4°C as indicated for each component.

The kit is intended for research use only. Sample preparation guidelines are provided, describing centrifugation for serum (clotting followed by centrifugation at 1,000 x g) and plasma (EDTA or heparin as anticoagulant, centrifuged at 1000 × g). Samples should be aliquoted and stored at -80°C, with repeated freeze-thaw cycles avoided. Additional sample-handling notes are included for urine, cerebrospinal fluid, cell culture supernatants, cell lysates, tissue homogenates, tissue lysates, and breast milk.