Chicken Melatonin ELISA Kit

A competitive ELISA from Assay Genie designed for the precise measurement of melatonin levels in chicken samples. The assay is coated with antigen and uses a colorimetric detection method, with absorbance read at 450 nm using a microplate reader. The kit demonstrates a sensitivity of 4.688 pg/mL and a measuring range of 7.813-500 pg/mL. It is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The protocol involves adding standard or sample with biotin-labeled antibody, incubation at 37°C, washing, TMB substrate development in the dark, and a stop reaction that changes the colour from blue to yellow. The kit should be stored sealed at 2-8°C and not cryopreserved.

Reported linearity for serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions falls within 82-105%. Recovery averages are 91% for serum, 96% for EDTA plasma, and 94% for heparin plasma. The assay is intended for research applications investigating melatonin biology in poultry, including its role in circadian rhythm regulation, the sleep-wake cycle, immune function, stress response, and antioxidant activity.