Chicken LIF/Leukemia inhibitory factor ELISA Kit

From Assay Genie, this sandwich ELISA kit is specifically designed for the quantitative detection of LIF (Leukemia Inhibitory Factor) levels in chicken serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. LIF is a multifunctional cytokine that plays a critical role in regulating cellular processes including cell differentiation, proliferation, and survival, and is known for its ability to inhibit the growth of leukemia cells.

The assay has a sensitivity of 37.5 pg/mL and a working range of 62.5-4000 pg/mL. It uses a sandwich ELISA double antibody format with colorimetric detection, and absorbance is measured at 450 nm. Performance has been validated by linearity testing on serum, EDTA plasma, and heparin plasma across 1:2, 1:4, and 1:8 dilutions, with recovery ranges of 87-104% (average 97%) for serum, 85-103% (average 94%) for EDTA plasma, and 86-105% (average 96%) for heparin plasma.

Kit components include a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody (100X), HRP-streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer (25X), plate sealer, and a technical manual. The associated Uniprot ID is F1N9H9. The kit should be stored at 2-8°C for 12 months.