Chicken IL-3 ELISA Kit (CHFI00043)

From Assay Genie, this high-quality Sandwich ELISA Kit (Double Antibody) is designed for the quantitative measurement of Chicken Interleukin-3 (IL-3) in chicken samples. It is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

The colorimetric assay has a sensitivity of 9.375 pg/mL with an assay range of 15.625–1000 pg/mL. Reported linearity values (n = 5) for serum at 1:2, 1:4 and 1:8 dilutions are 95-104%, 90-99% and 84-100%; for EDTA plasma 86-96%, 84-99% and 91-99%; and for heparin plasma 91-105%, 90-99% and 81-96%. Reported recovery ranges are 85-104% (average 95%) for serum, 87-103% (average 92%) for EDTA plasma, and 86-104% (average 97%) for heparin plasma.

The protocol uses a TMB substrate with absorbance read at 450 nm after adding stop solution, and kit components are stored at 2-8°C for 12 months.