Chicken IGFBP-3/Insulin Like Growth Factor Binding Protein 3 ELISA Kit

Designed by Assay Genie for the quantitative measurement of Insulin-like Growth Factor Binding Protein 3 (IGFBP-3) in chicken samples, this ELISA kit enables precise detection in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay is a competitive ELISA format coated with antigen, with a reported sensitivity of 3.75 ng/mL and a working range of 6.25-400 ng/mL. IGFBP-3 plays a key role in regulating insulin-like growth factor activity, influencing cell growth, differentiation, and survival, and its dysregulation has been linked to metabolic disorders and cancer.

Performance characteristics include linearity across 1:2, 1:4, and 1:8 dilutions: serum at 88-101%, 93-100%, and 90-97% respectively; EDTA plasma at 86-97%, 82-98%, and 90-101%; and heparin plasma at 83-97%, 81-96%, and 81-94% (n=10). Recovery rates are 90-103% (average 95%) for serum, 90-104% (average 99%) for EDTA plasma, and 91-104% (average 98%) for heparin plasma. The kit should be stored at 2-8°C (sealed) and not cryopreserved.