Chicken IGF-2/Insulin Like Growth Factor 2 ELISA Kit

Designed for the accurate quantification of IGF-2 (Insulin-like Growth Factor 2) levels in chicken samples, this Sandwich ELISA from Assay Genie uses a double-antibody format and is described as offering high sensitivity and specificity. The assay is suitable for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with absorbance measured at 450 nm.

The kit reports a sensitivity of 0.938 ng/mL and a detection range of 1.563-100 ng/mL. The procedure involves primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, and TMB colour development. Reported linearity for serum (n=5) is 83-97% (1:2), 86-99% (1:4) and 80-94% (1:8); for EDTA plasma 81-100%, 83-93% and 88-103%; and for heparin plasma 86-105%, 82-98% and 85-94%. Recovery ranges from 87-104% (serum, average 95%), 86-103% (EDTA plasma, average 93%) and 96-102% (heparin plasma, average 95%). Reagents are stored at 2-8°C for 12 months, protected from direct light where indicated.

IGF-2 is a key growth factor involved in regulating cell growth, proliferation and differentiation, with roles in embryonic development, tissue repair and metabolism. Its dysregulation has been linked to obesity, diabetes and certain cancers, highlighting its importance as a potential biomarker for studying these conditions and exploring therapeutic interventions.