Chicken IFN gamma DIY ELISA Kit

This DIY ELISA kit from Assay Genie enables quantitative measurement of chicken interferon gamma (IFN-γ), a key Th1-type cytokine central to cell-mediated immunity, macrophage activation, and antiviral defence in avian species. The format is widely applied in poultry immunology research, including studies of vaccine efficacy, Marek's disease, infectious bursal disease, avian influenza, and stress-induced immunosuppression in broilers and laying hens.

The kit ships as a bulk DIY pack from Assay Genie, providing capture antibody, biotinylated detection antibody, recombinant chicken IFN-γ standard, streptavidin-HRP conjugate, wash buffer, TMB substrate, and stop solution sufficient for up to 10 microplates. Researchers coat their own plates, which gives flexibility to choose plate density, antibody pairing, and standard curve layout. The chicken IFN-γ capture and detection antibodies show no significant cross-reactivity with mammalian IFN-γ homologues, keeping readouts avian-specific.

For best stability, store unopened reagents at -20 °C and keep the reconstituted standard in single-use aliquots to limit freeze-thaw cycles. The kit is supplied for research use only and is intended for in vitro quantification of IFN-γ in chicken-origin cell culture supernatant, splenocyte lysate, or mucosal wash samples in immunological studies.