Chicken FT4 (Free Thyroxine) ELISA Kit

Developed by Assay Genie for poultry research and veterinary applications, this Competitive ELISA Kit is specifically designed for the accurate measurement of free thyroxine (FT4) levels in chicken samples. Free thyroxine is a crucial hormone that regulates metabolism, growth, and development in chickens, and abnormal levels can serve as an important biomarker for studying thyroid function and potential therapies for thyroid disorders. The assay is run in a competitive ELISA format coated with antigen and uses a colorimetric detection method.

The kit has a sensitivity of 0.938 pg/mL and an assay range of 1.563-100 pg/mL. It accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. According to the manufacturer, kits should be stored sealed at 2-8°C and should not be cryopreserved.

Reported linearity values (n=10) are: serum 89-101% at 1:2, 86-103% at 1:4, and 86-103% at 1:8; EDTA plasma 94-99%, 88-99%, and 82-94% at the same dilutions; and heparin plasma 81-97%, 85-98%, and 80-94%. Recovery ranges and averages (n=10) are: serum 85-103% (94% average); EDTA plasma 89-103% (98% average); and heparin plasma 87-103% (96% average).