Chicken DHT (Dihydrotestosterone) ELISA Kit (CHFI00107)

This competitive immunoassay from Assay Genie quantifies dihydrotestosterone in avian samples, supporting research in poultry reproductive endocrinology, sexual differentiation, and steroid hormone metabolism. DHT is a potent androgen produced from testosterone via 5α-reductase activity, and its measurement in chickens provides insight into gonadal function, breeding performance, and hormonal regulation in avian models.

Configured as a microplate-format ELISA kit, the assay provides 96 Assays of reagents suitable for standard curves, internal controls, and multiple experimental replicates in a single workflow. The kit is validated for chicken samples, and the protocol follows a competitive binding principle in which analyte in the specimen competes with conjugated antigen for a limited number of antibody binding sites, with signal inversely related to analyte concentration.

Researchers should store the kit components refrigerated and equilibrate reagents to room temperature before use to ensure reproducible performance across the plate. The optical readout is measured on a standard microplate reader, and the procedure is intended for research use only in laboratory investigations of avian endocrinology, hatchery management studies, and reproductive physiology research.