Chicken CCAAT/enhancer-binding protein beta (CEBPB) ELISA Kit

The Chicken CEBPB ELISA Kit from Assay Genie is a Sandwich-format immunoassay built on a pre-coated 96-well microplate for quantitative measurement of CCAAT/enhancer-binding protein beta in chicken serum and plasma samples. Detection is colorimetric, typically read at 450 nm, with a standard curve included for accurate interpolation of CEBPB concentrations.

CEBPB (C/EBPβ) is a transcription factor implicated in inflammatory signaling, adipogenesis, and hepatic acute-phase responses, supporting its use in poultry immunology, nutrition, and stress-response research. The assay is intended for research use only and for in vitro applications.

Each kit contains a pre-coated microplate, standards, detection antibody, and the reagents required to run the full 96-well plate. Unopened kits should be stored refrigerated at 2–8 °C; reconstituted components are typically aliquoted and stored frozen to limit freeze-thaw cycles. Bring all reagents to room temperature before use.

Assay Genie provides a complete buffer set and wash concentrate alongside the coated plate, supporting reproducible quantification across chicken serum and plasma matrices. The Sandwich ELISA format pairs a capture antibody pre-bound to the wells with a biotinylated detection antibody, producing signal proportional to CEBPB concentration over the standard curve's dynamic range. Typical runs require only 50–100 µl of sample per well, and standard dilutions generate the reference curve used for interpolation.

For poultry researchers, CEBPB expression shifts are monitored during bacterial and viral challenge studies, feed-restriction trials, and metabolic profiling of liver tissue. The kit's colorimetric readout at 450 nm is compatible with most benchtop microplate readers, and the 96-well plate layout allows processing of multiple conditions, time points, or treatment groups in parallel. Reconstituted standards and detection reagents should be aliquoted and frozen at -20 °C to preserve activity across repeated freeze-thaw cycles, while the sealed microplate is stored refrigerated at 2–8 °C until first use.

This research-use-only assay is intended for in vitro measurement of endogenous chicken CEBPB and is not validated for diagnostic or therapeutic procedures.