Chicken C3/Complement Component 3 ELISA Kit

This Sandwich ELISA kit from Assay Genie is designed for the precise measurement of complement component 3 (C3) levels in chicken samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. Using a double antibody sandwich format with colorimetric detection, the kit delivers high sensitivity and specificity for research applications investigating the role of C3 in immune response, inflammation, and defense against pathogens.

The assay has a sensitivity of 1.875 ng/mL and a working range of 3.125-200 ng/mL. Recovery has been validated in serum (range 92-103%, average 97%), EDTA plasma (range 89-100%, average 94%), and heparin plasma (range 86-105%, average 91%). Linearity was assessed across 1:2, 1:4, and 1:8 serial dilutions in these matrices, with serum showing 93-104%, 86-103%, and 86-102% respectively. The protocol uses a biotin-labeled detection antibody and HRP-Streptavidin conjugate, with TMB substrate development and absorbance reading at 450 nm. Standard storage is at 2-8°C for 12 months. Complement component 3 is a key biomarker linked to infections, autoimmune disorders, and inflammatory diseases, making this kit a reliable tool for studying chicken health and disease.