Chicken BMP2 ELISA Kit

Assay Genie's Chicken BMP2 ELISA Kit is a solid phase Sandwich ELISA designed for the quantitative detection of Bone Morphogenetic Protein 2 (BMP-2) in chicken samples. BMP-2 is described as a key signaling molecule involved in bone and cartilage development, playing a crucial role in regulating cell differentiation and tissue growth, with dysregulation implicated in various skeletal disorders. The kit uses a double antibody sandwich format with colorimetric detection and is intended for research applications in bone and cartilage biology.

The assay has a sensitivity of 18.75 pg/mL and a working range of 31.25-2000 pg/mL. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reagents should be stored at 2-8°C for 12 months. The associated UniProt ID is Q90751, and the target is also known by the synonyms BMP-2, Bone Morphogenetic Protein 2, BMP2A, and BMP-2A.

Validation data demonstrate strong performance across matrices. Linearity at 1:2, 1:4, and 1:8 dilutions ranged from 87-100%, 93-105%, and 88-102% for serum (n=5); 84-100%, 82-95%, and 84-99% for EDTA plasma (n=5); and 80-97%, 82-97%, and 81-94% for heparin plasma (n=5). Recovery ranged from 90-103% (average 97%) for serum, 87-99% (average 93%) for EDTA plasma, and 89-104% (average 99%) for heparin plasma. The product is shipped at +4°C.