CDK8 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit provides a colorimetric indirect ELISA method for detecting CDK8 protein expression directly in cultured cells. The assay is lysate-free and supports high-throughput screening of relative CDK8 levels, including evaluation of treatments, siRNA, chemicals, or activators. It is designed for adherent cells and is reactive with human and mouse samples, with a stated dynamic range of > 5000 Cells and readout at 450 nm. Storage is specified at 4°C for 6 Months.

CDK8-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. For normalization, a monoclonal anti-human GAPDH antibody is included as an internal positive control, and a Crystal Violet whole-cell staining step is used to determine cell density, allowing absorbance values to be normalized to cell amounts. CDK8 (Gene ID: 1024; UniProt ID: P49336) is a member of the cyclin-dependent kinase family involved in regulation of gene expression and cell cycle progression, and the kit is classified for Epigenetics and Nuclear Signaling research.