CDC6 (Phospho-Ser54)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit provides colorimetric detection at 450 nm of CDC6 phosphorylated at serine 54 in cultured cells. The kit is designed for use with adherent cell samples and is reactive with human and mouse specimens, with a stated dynamic range of "> 5000 Cells." It uses an indirect ELISA format in which CDC6 (Phospho-Ser54) is captured by phospho-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition.

The qualitative workflow is lysate-free and high-throughput, making it suitable for measuring relative levels of phosphorylated CDC6 in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on CDC6 phosphorylation. Two normalization methods are included: a monoclonal anti-GAPDH antibody acts as an internal positive control for target absorbance, and a Crystal Violet whole-cell staining step is used to determine cell density, allowing absorbance values to be adjusted to plating differences.

CDC6 is a key regulator of DNA replication, playing a crucial role in cell cycle progression and DNA synthesis. Phosphorylation of CDC6 at serine 54 has been shown to impact its function, making this phospho-specific assay a relevant tool for cell cycle research, including studies in cancer research and other fields.