CDC6 Colorimetric Cell-Based ELISA Kit

Designed for the precise measurement of CDC6 in cell lysates and cell culture supernatants, this Cell-Based ELISA Kit from Assay Genie provides a lysate-free, high-throughput assay for the relative quantification of CDC6 expression in cultured adherent cells. The kit uses an indirect ELISA format in which CDC6 is captured by target-specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction, measured at 450 nm. It has been validated for human and mouse reactivity with a dynamic range of > 5000 cells, and is supplied in a 96-Well Microplate format suited to qualitative profiling under varied treatment conditions, including siRNA or chemical modulators.

Two normalization strategies are supported: a monoclonal anti-human GAPDH antibody supplied as an internal positive control, and Crystal Violet whole-cell staining to determine cell density and adjust for plating differences. The kit is intended for research into cell cycle regulation, DNA replication and cell division, with storage at 4 °C and a stated stability of 6 months. Relevant database identifiers for the target include Gene ID 990 and UniProt ID Q99741, with the encoded protein reported at a molecular weight of 62,720 Da.