CDC25C (Phospho-Thr48)Fluorometric Cell-Based ELISA Kit

This Fluorometric Cell-Based ELISA Kit from Assay Genie is designed to monitor phosphorylation of CDC25C at Thr48 in cultured cells. The lysate-free, high-throughput workflow uses an indirect ELISA format in which CDC25C (Phospho-Thr48)-specific primary antibodies are detected with Dye 1- and Dye 2-conjugated secondary antibodies, enabling fluorometric readout at Ex/Em 651/667 and 495/521. The kit is suitable for measuring relative levels of phosphorylated CDC25C in cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on CDC25C phosphorylation.

For data normalization, a monoclonal anti-human GAPDH antibody is included as an internal positive control to normalize target RFU values, and a separate antibody against the non-phosphorylated counterpart of CDC25C (Phospho-Thr48) is provided so that the RFU signal for phosphorylated CDC25C can be normalized against total CDC25C. The assay is intended for cell samples, has a dynamic range of > 5000 Cells, is reactive with human samples, and is stored at 4°C for 6 months. The target corresponds to Gene ID 995, UniProt ID P30307, and OMIM entry 157680.