CDC25B (Phospho-Ser353)Colorimetric Cell-Based ELISA Kit

Assay Genie's Cell-Based ELISA Kit is specifically designed for the accurate and sensitive detection of phosphorylated CDC25B at the serine 353 residue in cell lysates. The colorimetric assay uses an indirect ELISA format in which HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition, with absorbance read at 450 nm. The kit offers high specificity and reproducibility and is suitable for adherent cells.

Configured as two 96-well microplates, the assay is lysate-free and high-throughput, enabling measurement of relative amounts of phosphorylated CDC25B in cultured cells. It can be used to screen the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on CDC25B phosphorylation. Reactivity is human, with a dynamic range of > 5000 Cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising absorbance values, and Crystal Violet whole-cell staining is provided for determining cell density.

CDC25B is a key regulator of cell cycle progression and is involved in promoting cell proliferation. Phosphorylation at serine 353 is crucial for its activity and function, making the kit relevant to studies of cell cycle regulation and potential therapeutic interventions.