CD71/TfR (Phospho-Ser24)Colorimetric Cell-Based ELISA Kit

An In-Cell ELISA kit from Assay Genie is designed to detect CD71/TfR protein phosphorylation and expression in cultured cells, offering a lysate-free, high-throughput assay for measuring relative levels of phosphorylated CD71/TfR (Phospho-Ser24) in cell samples. The assay uses an indirect ELISA format in which CD71/TfR (Phospho-Ser24) is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. Colorimetric detection is performed at 450 nm.

The kit can be used to measure relative amounts of phosphorylated CD71/TfR in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on CD71/TfR phosphorylation. Adherent cells are the indicated sample type. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density so that absorbance values can be adjusted for plating differences.

According to the manufacturer, the kit is reactive with human and mouse samples and supports a dynamic range of greater than 5000 cells. The target is CD71/TfR (Phospho-Ser24); the associated gene symbol is TFR1, and the encoded transferrin receptor protein has a molecular weight of 84,871 Da.