CD3 zeta (Phospho-Tyr142)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the qualitative detection of CD3 zeta phosphorylated at tyrosine 142 in cultured cell samples, supporting research into T-cell signaling, immune responses and cancer immunotherapy. Detection is performed by a colorimetric readout at 450 nm, using an indirect ELISA format in which phospho-specific primary antibodies capture the target and HRP-conjugated secondary antibodies drive a colorimetric reaction upon substrate addition.

The kit has been validated for human, mouse and rat reactivity with a stated dynamic range of > 5000 cells, and is intended for use with adherent cells. A monoclonal anti-GAPDH antibody is included as an internal positive control, and Crystal Violet whole-cell staining can be used to normalize absorbance readings to cell density. The target is encoded by CD247 (Gene ID: 919; UniProt ID: P20963; molecular weight 18,696 Da), which produces the T-cell surface glycoprotein CD3 zeta chain, a key component of the T-cell receptor complex that couples antigen recognition to intracellular signal-transduction pathways.