CBL Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit provides a lysate-free, high-throughput format for the qualitative detection of CBL protein expression in cultured adherent cells. Detection is achieved through an indirect ELISA approach in which CBL-specific primary antibodies are bound by HRP-conjugated secondary antibodies, enabling a colorimetric reaction upon substrate addition. The kit supports measurement of relative CBL levels and can be used to screen the effects of treatments, inhibitors, or activators on CBL expression.

Key specifications include reactivity with Human, Mouse, and Rat samples, a colorimetric detection method at 450 nm, and a dynamic range of > 5000 Cells. The assay is provided in a 96-Well Microplate format and is stored at 4 °C with a stated stability of 6 Months. Two normalisation strategies are incorporated: a monoclonal anti-GAPDH antibody acts as an internal positive control for target absorbance, and a Crystal Violet whole-cell staining step is used to determine cell density and adjust for plating differences.

CBL (Gene ID: 867; UniProt ID: P22681; OMIM: 165360) is an E3 ubiquitin-protein ligase with a molecular weight of 99,633 Da that functions as a negative regulator of multiple signalling pathways initiated by activated cell surface receptors. The RING finger protein mediates ubiquitin transfer from E2 ubiquitin-conjugating enzymes to specific substrates, targeting them for proteasomal degradation.