Caspase 9 (Phospho-Tyr153)Colorimetric Cell-Based ELISA Kit

Designed to measure phosphorylated tyrosine 153 levels in Caspase-9 in cell lysates, this Colorimetric Cell-Based ELISA Kit from Assay Genie offers high sensitivity and specificity for apoptosis research. The cell-based format operates without the need for lysate preparation and supports high-throughput screening of treatments, inhibitors, or activators on Caspase 9 phosphorylation in cultured human cells.

The assay uses an indirect ELISA format: primary antibodies specific for Caspase 9 (Phospho-Tyr153) capture the target, and HRP-conjugated secondary antibodies enable colorimetric detection at 450 nm. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, while a Crystal Violet whole-cell staining step allows cell density to be determined and plating differences to be adjusted. The kit uses a 96-well microplate format and is intended for adherent cells, with a dynamic range of > 5000 Cells. The target gene is CASP9 (Gene ID: 842; UniProt ID: P55211), and the research area is cell death.