Caspase 9 (Phospho-Thr125)Colorimetric Cell-Based ELISA Kit

A colorimetric cell-based ELISA kit from Assay Genie for the detection of Caspase 9 phosphorylated at Thr125 in cell lysates. The assay uses an indirect ELISA format: phospho-Thr125 Caspase 9 is captured by specific primary antibodies, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction read at 450 nm.

The kit is designed for adherent cell samples, with reactivity reported for human, mouse, and rat, and a stated dynamic range of > 5000 cells. It is described as a convenient, lysate-free, high-throughput format suitable for measuring relative levels of phosphorylated Caspase 9 in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on Caspase 9 phosphorylation. Two normalization methods are included: an anti-GAPDH monoclonal antibody as an internal positive control, and Crystal Violet whole-cell staining for cell density determination.

Caspase-9 is a cysteine-aspartic acid protease (gene symbol CASP9) that plays a central role in the execution phase of cell apoptosis; phosphorylation at Thr125 is reported to modulate its activity. The kit is intended for research into apoptotic pathways, cell signaling, and conditions such as cancer and neurodegenerative disorders, where dysregulation of apoptosis is relevant.