Caspase 9 (Phospho-Ser196)Colorimetric Cell-Based ELISA Kit

A Colorimetric Cell-Based ELISA Kit from Assay Genie designed for the qualitative detection of Caspase 9 phosphorylation at Serine 196 in cultured human cells. The assay targets the phospho-Ser196 epitope of CASP9 (Gene ID: 842; UniProt ID: P55211) and is suited to adherent cell samples, making it applicable to research into apoptosis and programmed cell death signaling.

Detection uses an indirect ELISA format, in which a phospho-specific primary antibody captures the target and an HRP-conjugated secondary antibody catalyses a colorimetric reaction read at 450 nm. The dynamic range supports measurements above 5000 cells. A monoclonal anti-GAPDH antibody is included as an internal positive control, and Crystal Violet whole-cell staining can be used to normalise absorbance readings to cell density, allowing correction for plating differences across wells.

The kit is intended for research applications such as screening the effects of treatments, inhibitors (including siRNA or chemicals), and activators on Caspase 9 phosphorylation. As a phospho-specific, cell-based ELISA in the cell death research area, it provides a high-throughput, lysate-free approach to investigating the regulation of caspase-9 activity within apoptotic pathways.