Caspase 6 (Phospho-Ser257)Colorimetric Cell-Based ELISA Kit

This Colorimetric Cell-Based ELISA Kit from Assay Genie is designed to measure levels of phosphorylated Caspase-6 at Ser257 in cell lysates, providing a tool for studying apoptosis and programmed cell death pathways. The assay targets Caspase 6 (Phospho-Ser257) and is reactive with Human, Mouse, and Rat samples, with a stated dynamic range of > 5000 Cells and colorimetric detection at 450 nm. The kit can be used to measure the relative amounts of phosphorylated Caspase 6 in cultured cells and to screen for the effects of various treatments, inhibitors, or activators on Caspase 6 phosphorylation.

Detection is performed using an indirect ELISA format: primary antibodies specific for Caspase 6 (Phospho-Ser257) are captured, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is supplied as an internal positive control, and Crystal Violet whole-cell staining can be used after the colorimetric readout to assess cell density, allowing absorbance values to be normalized to cell number. The kit is described as lysate-free and high throughput, with protocols suitable for adherent cells, and is positioned for use in drug discovery, cancer research, and cell biology studies of cell death and survival.