Caspase 3 (Phospho-Ser150)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed to detect phosphorylated Caspase 3 (Phospho-Ser150) in cultured cells. The assay uses an indirect ELISA format in which a target-specific primary antibody is captured and detected with an HRP-conjugated secondary antibody, producing a colorimetric reaction upon substrate addition at 450 nm. The kit is described as convenient and lysate-free, supporting high throughput screening of treatments, inhibitors (such as siRNA or chemicals), and activators that affect Caspase 3 phosphorylation.

Reactivity is reported for Human, Mouse, and Rat samples, with a dynamic range of > 5000 Cells. To account for the qualitative nature of the assay, two normalization methods are provided: a monoclonal anti-GAPDH antibody serves as an internal positive control for normalizing target absorbance, and a Crystal Violet whole-cell staining step can be used to determine cell density and adjust for plating differences. The Caspase 3 (Phospho-Ser150) target is catalogued under gene symbol CASP3 (Gene ID: 836, UniProt ID: P42574).