Casein Kinase II alpha Colorimetric Cell-Based ELISA Kit

Designed for the precise measurement of Casein Kinase II alpha in cultured cells, this colorimetric cell-based ELISA kit from Assay Genie offers sensitive and specific detection of the Casein Kinase II alpha protein expression profile. The assay is lysate-free and suited to high-throughput workflows, allowing researchers to quantify relative target levels and to screen for the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on the target.

The kit uses an indirect ELISA format: Casein Kinase II alpha is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition at 450 nm. Because the assay is qualitative, an anti-human GAPDH monoclonal antibody is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step can be used to determine cell density and correct for plating differences.

Reactivity is reported for human, mouse, and rat samples, with a dynamic range of > 5000 cells. The kit is supplied in a 96-well microplate format and is intended for storage at 4°C for 6 months. Casein Kinase II alpha is a key regulatory enzyme involved in cell cycle regulation, gene expression, and signal transduction, and its dysregulation has been linked to cancer, neurodegenerative disorders, and inflammation, making it a relevant target for drug discovery research.