Canine TNF alpha DIY ELISA Kit

The Canine TNF alpha DIY ELISA Kit from Assay Genie provides researchers with the core reagents needed to assemble their own microplate-based immunoassay for quantifying tumor necrosis factor alpha in canine samples. TNF alpha is a pro-inflammatory cytokine central to innate immune signaling, and accurate measurement in dogs supports research into inflammatory disease models, vaccine response, infectious disease, and comparative immunology studies.

This DIY format ships the capture antibody, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, and wash and stop buffers separately, allowing the user to coat and block plates according to their experimental throughput. Researchers typically load 100 uL of diluted standard or sample per well, and the full protocol can be completed in roughly half a working day.

Unopened kit components should be stored refrigerated and protected from light, with reconstituted standards held in single-use aliquots to avoid freeze-thaw degradation. The kit is intended for research use only in veterinary and biomedical applications.