Canine PD-L1 DIY ELISA Kit

The Canine PD-L1 DIY ELISA Kit from Assay Genie supports quantitative measurement of programmed death-ligand 1 in canine research samples. PD-L1 is an immune checkpoint protein expressed on tumor cells and antigen-presenting cells, and its expression in dogs is actively investigated in comparative oncology studies of melanoma, osteosarcoma, mast cell tumors, and hemangiosarcoma.

This DIY-format microplate kit provides researchers with the core reagents, antibodies, and buffers needed to assemble and optimize their own immunoassay workflow on standard well plates, offering flexibility in coating conditions, incubation times, and sample types beyond what a fully pre-configured kit permits. The format supports up to 10 microplates depending on plate layout and reagent consumption per run.

Kit components should be stored refrigerated at 4 degrees C upon arrival, with freeze-thaw cycles of any working antibody or standard solutions minimized to preserve binding activity. Reconstituted standards and concentrated detection reagents are commonly aliquoted into single-use vials for long-term frozen storage at -20 degrees C or lower. Standard wash buffer, blocking buffer, and chromogenic substrate are commonly prepared from the supplied concentrates.

Intended for research use only and in vitro applications, this Assay Genie kit is applicable to academic and translational laboratories working on canine immunotherapy biomarkers, checkpoint inhibitor response prediction, and veterinary comparative immunology. Each lot is quality-controlled for antibody affinity and lot-to-lot reproducibility, and the reagent volumes are scaled to accommodate exploratory pilot studies as well as larger longitudinal cohorts in canine clinical trials.