Canine N-Terminal Pro Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit

This Assay Genie canine NT-ProBNP ELISA Kit is intended for the quantitative measurement of N-Terminal Pro Brain Natriuretic Peptide in canine specimens, supporting veterinary cardiology research into heart failure, dilated cardiomyopathy, and congestive cardiac dysfunction. The kit quantifies a stable cleavage fragment of proBNP that circulates at elevated concentrations in dogs with myocardial stretch and volume overload, making it a useful biomarker for clinical investigation and preclinical studies of canine cardiovascular disease.

Each kit contains a pre-coated 96-well microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, and assay buffers sufficient for 96 determinations. The protocol uses a 50 µL sample volume with serum or plasma as the preferred matrix, and the standard curve is read on a standard microplate reader. Unopened kit components should be stored refrigerated at 2-8°C; reconstituted standards should be aliquoted and frozen at -20°C, with limited freeze-thaw cycles recommended to preserve antibody binding activity.

For research use only, this species-specific canine assay is intended for laboratory investigation of cardiac biomarkers in dogs and is not a substitute for veterinary diagnostic imaging, echocardiography, or clinical examination.