Canine Methylmalonic Acid (MMA) ELISA Kit

This competitive ELISA kit from Assay Genie is intended for research use only and is designed for canine studies. The manufacturer reports a measurement range of 0.312-20 ng/mL. According to the product description, the assay quantifies methylmalonic acid levels in canine serum, plasma, and urine samples. Methylmalonic acid is described as a key biomarker for assessing cobalamin (vitamin B12) deficiency in dogs, with elevated levels potentially indicating such a deficiency. The manufacturer notes that monitoring methylmalonic acid levels can aid in the early detection and management of cobalamin deficiency, which is reported to be commonly seen in certain dog breeds and gastrointestinal disorders.

The assay protocol involves adding 50µL of standard, blank, or sample per well, followed by incubation with detection reagents at 37°C and a substrate step. Optical density is determined at 450 nm using a microplate reader. The manufacturer provides sample preparation guidance for serum, plasma, urine, cerebrospinal fluid, cell culture supernatant, cell lysates, tissue homogenates, tissue lysates, and breast milk.