Canine Macrophage Inflammatory Protein 3 Beta (MIP3b) ELISA Kit

The Assay Genie canine MIP-3β (also termed CCL19) chemokine quantification kit supports veterinary immunology research on lymphoid homing, dendritic cell trafficking, and T-cell recruitment in dogs. The colorimetric sandwich ELISA measures MIP-3β across a standard curve of 15.6 pg/mL to 1000 pg/mL with a sensitivity below 5.6 pg/mL, using a 100 µL sample volume per well. Researchers investigating canine lymphoma, chronic inflammatory disorders, vaccine responses, or comparative chemokine signaling can apply serum, plasma, or other biological fluids prepared according to the supplied protocol. Reagents should be kept refrigerated upon arrival; allow the pre-coated microplate to equilibrate to room temperature before running the assay, and avoid repeated freeze-thaw cycles of reconstituted standards to preserve antibody binding activity. The kit contains a 96-well microplate, lyophilized recombinant canine MIP-3β standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, TMB substrate, stop solution, and a technical manual with plate-layout templates. Capture and detection antibodies are raised against the canine CCL19 sequence, providing species-specific recognition without significant cross-reactivity to related chemokines such as CCL20 or CCL21.