Canine Interleukin 1 Beta (IL-1b) ELISA Kit for

This canine-specific interleukin-1 beta ELISA kit from Assay Genie quantifies IL-1β across serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants using a sandwich immunoassay. The 96-well microplate format delivers colorimetric readout serving veterinary immunology laboratories and academic canine research programs.

Validated Performance:

• Standard curve range: 15.6 to 1000 pg/mL
• Sensitivity: less than 4.7 pg/mL
• Required sample volume: 50 µL per well
• Total incubation time: approximately 3.5 hours

IL-1β is a pro-inflammatory cytokine implicated in canine osteoarthritis, periodontal disease, sepsis models, and vaccine adjuvant response studies. Reagents are shipped refrigerated on cold packs; reconstituted standards and detection antibody require storage at -20°C, while unused strip wells remain stable at 2-8°C. The kit contains a pre-coated plate, lyophilized recombinant canine IL-1β standard, biotinylated detection antibody, streptavidin-HRP, wash buffer concentrate, TMB substrate, and stop solution.

For research use only; not validated for clinical diagnostics.