Canine IL-2 ELISA Kit (CNFI00025)

From Assay Genie, this sandwich ELISA kit enables quantitative measurement of canine interleukin-2 (IL-2), a key cytokine involved in T-cell activation and immune regulation. Using a double-antibody sandwich ELISA format read colorimetrically at 450 nm, the assay has a sensitivity of 9.375 pg/mL and a quantitative range of 15.625-1000 pg/mL.

The kit is validated for use with canine serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reagents should be stored at 2-8°C for 12 months. Recovery in spiked samples ranges from 87-103% in serum (average 96%), 89-105% in EDTA plasma (average 96%), and 88-103% in heparin plasma (average 97%). Linearity across 1:2, 1:4, and 1:8 dilutions spans 80-105% across all three sample matrices.

The procedure involves a 90-minute primary incubation at 37°C, 60-minute biotin-labeled detection antibody binding, 30-minute HRP-streptavidin incubation, and 10-20 minutes of TMB substrate development in the dark before stop solution is added and absorbance read. IL-2 (synonyms: Interleukin 2, IL2, TCGF, Lymphokine; UniProt Q29416) is studied in contexts including infection, vaccination, and autoimmune disorders, supporting veterinary immunology research applications.