Canine IL-17A DIY ELISA Kit

A flexible immunoassay for veterinary immunology laboratories, this do-it-yourself kit enables researchers to quantify canine interleukin-17A with full control over plate preparation. IL-17A is a pro-inflammatory cytokine secreted by Th17 cells that mediates mucosal immunity and inflammatory pathology, making it a target of interest in canine studies of dermatitis, periodontal disease, inflammatory bowel conditions, and vaccine response.

Assay Genie provides the capture antibody, biotinylated detection reagent, recombinant canine IL-17A standard, enzyme conjugate, and supporting buffers as separate vial components for self-coating onto microplates. Reagent volumes are sufficient for up to 10 plates, accommodating both small pilot experiments and larger longitudinal screening campaigns.

The DIY configuration supports research use only applications where laboratories need to optimize coating conditions, adapt plate formats, or stretch reagent usage across multiple study cohorts. Researchers can pair the assay with cell culture supernatants from stimulated canine peripheral blood mononuclear cells to profile Th17 responses, or apply it to clarify how candidate therapeutics modulate cytokine output in canine inflammatory models.