Canine IL-1 alpha DIY ELISA Kit

The Canine IL-1 alpha DIY ELISA Kit from Assay Genie is supplied in a do-it-yourself format that lets investigators coat their own microplates with the supplied anti-canine IL-1 alpha capture antibody. This is a canine-specific immunoassay for the pro-inflammatory cytokine interleukin-1 alpha, with no significant cross-reactivity reported for related canine cytokines or for IL-1 beta.

The kit reagents support up to 10 plates and the assay is read via absorbance measurement on a standard microplate reader. Each pack includes the capture antibody, biotinylated detection antibody, recombinant canine IL-1 alpha standard, streptavidin-HRP conjugate, TMB substrate, wash buffer, and assay diluent. Components should be kept refrigerated upon arrival; the lyophilized standard should be stored frozen for long-term stability, and the TMB substrate should be protected from light.

Researchers coat plates overnight at 4 degrees C, then perform the standard ELISA washing and incubation workflow. The format is suited to veterinary immunology research, inflammatory disease studies, and vaccine response work in dogs, and is compatible with cell culture supernatant, serum, plasma, and tissue homogenate samples.